Alisha, 30, was seen touching the knee of singer Justin — before he took hold of her hand.
The pics blew up the internet, and everyone was looking to Justin for answers.
“At one stage he grabbed her hand and rested it on his knee. She then gently started stroking his leg.
“Then he clutched her hand with both of his and was playing with her hands.”
Justin had been snapped wearing his wedding ring earlier in the week but was not seen with it on during the night out.
The pair, currently filming American football drama film Palmer, were part of a small group out drinking at The Absinthe House, on Bourbon Street, in New Orleans on Thursday.
But they were alone on a balcony for around 40 minutes, before going inside. It is believed they left via a rear exit around 12.30am.
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Alisha is best known for her role as Maia Roberts in the television series Shadowhunters, and can currently be seen starring alongside Michael B Jordan in the Netflix series Raising Dion.
Justin and Biel, who wed in 2012, are widely considered to have one of the most rock-solid marriages in Hollywood.
Both regularly post heartfelt videos on Instagram.
Justin has since posted an emotional apology about the pictures, saying he made a “strong lapse in judgement”.
In a grovelling Instagram post, he wrote: “I stay away from gossip as much as I can, but for my family, I feel it is important to address recent rumors that are hurting the people I love.
“A few weeks ago I displayed a strong lapse in judgment — but let me be clear — nothing happened between me and my costar.
“I drank way too much that night and I regret my behavior. I should have known better.”
The ex-NSYNC star added: “This is not the example I want to set for my son.
“I apologize to my amazing wife and family for putting them through such an embarrassing situation, and I am focused on being the best husband and father I can be.
“This was not that. I am incredibly proud to be working on Palmer.
“Looking forward to continuing to make this movie and excited for people to see it.”